ELRIG-Forum 2018: Abstracts
Susanne Müller-Knapp, SGC Oxford, UK
The SGC (Structural Genomics Consortium) is an academic organisation with laboratories at the universities of Oxford (UK), Toronto (Canada), North Carolina US) Campinas (Brazil) and Frankfurt (Germany). The consortium encompasses a large public-private partnership involving currently nine pharmaceutical companies.
Wojciech Senkowski,Uppsala University, Sweden
Cancer cells grown as two-dimensional (2D) monolayer cell cultures have been widely applied for research in cancer biology and anticancer drug discovery. However, 2D cultures do not closely simulate the complex microenvironment and tissue context of in vivo solid tumors. Using three-dimensional (3D) cell cultures, such as multicellular tumor spheroids (MCTS) has been proposed to address some of these limitations.
Hauke Cornils and Stephen Hess, Evotec, Hamburg, Germany
The TRPM4 ion channel is a member of the Transient Receptor Potential Melastatin family, and is permeable to monovalent cations but impermeable to calcium. Deletion of this gene in mice ameliorates the course of disease progression in an animal model of MS. To find a specific inhibitor of TRPM4 channels to further define their role in MS, we screened a library of 250K small drug-like molecules using cells expressing human TRPM4 channels with a membrane potential dye readout.
Sabine Lange, Cellular Dynamics, Madison, USA
Human induced pluripotent stem cell (iPSC) technology offers a solution by providing an unprecedented access to biologically relevant human cell types in essentially unlimited supply. Human iPSC derived cells that can recapitulate native behavior and are amenable to large-scale scientific manipulation offer a new and highly relevant model for phenotypic screening and target identification.
Martin Hoffmann, Merck, Darmstadt, Germany
In recent times mass spectrometry detection was established as label-free alternative for the detection of substrates and products in enzyme reaction, which were run in high throughput screening. Advances in hardware improvements for MALDI-TOF in terms of automation and measuring time made it now possible to integrate the mass spectrometer as a read-out detection method in high throughput screening.
